ID   RU1A_HUMAN     STANDARD;      PRT;   282 AA.
AC   P09012;
DT   01-NOV-1988 (REL. 09, CREATED)
DT   01-NOV-1988 (REL. 09, LAST SEQUENCE UPDATE)
DT   01-OCT-1996 (REL. 34, LAST ANNOTATION UPDATE)
DE   U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A (U1 SNRNP A PROTEIN).
GN   SNRPA.
OS   HOMO SAPIENS (HUMAN).
OC   EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA;
OC   EUTHERIA; PRIMATES.
RN   [1]
RP   SEQUENCE FROM N.A.
RC   TISSUE=LIVER;
RX   MEDLINE; 91340152.
RA   NELISSEN R.L.H., SILLEKENS P.T.G., BEIJER R.P.,
RA   GEURTS VAN KESSEL A.H.M., VAN VENROOIJ W.J.;
RL   GENE 102:189-196(1991).
RN   [2]
RP   SEQUENCE FROM N.A.
RX   MEDLINE; 88111575.
RA   SILLEKENS P.T.G., HABETS W.J., BEIJER R.P., VAN VENROOIJ W.J.;
RL   EMBO J. 6:3841-3848(1987).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-95.
RX   MEDLINE; 91061907.
RA   NAGAI K., OUBRIDGE C., JESSEN T.-H., LI J., EVANS P.R.;
RL   NATURE 348:515-520(1990).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS).
RX   MEDLINE; 95075454.
RA   OUBRIDGE C., ITO N., EVANS P.R., TEO C.-H., NAGAI K.;
RL   NATURE 372:432-438(1994).
RN   [5]
RP   STRUCTURE BY NMR OF 11-94.
RX   MEDLINE; 91172834.
RA   HOFFMAN D.W., QUERY C.C., GOLDEN B.L., WHITE S.W., KEENE J.D.;
RL   PROC. NATL. ACAD. SCI. U.S.A. 88:2495-2499(1991).
RN   [6]
RP   STRUCTURE BY NMR OF 1-102.
RX   MEDLINE; 94349935.
RA   HOWE P.W.A., NAGAI K., NEUHAUS D., VARANI G.;
RL   EMBO J. 13:3873-3881(1994).
RN   [7]
RP   STRUCTURE BY NMR OF 2-102.
RX   MEDLINE; 96186818.
RA   ALLAIN F.H.-T., GUBSER C.C., HOWE P.W.A., NAGAI K., NEUHAUS D.,
RA   VARANI G.;
RL   NATURE 380:646-650(1996).
RN   [8]
RP   STRUCTURE BY NMR OF 1-117.
RX   MEDLINE; 96180024.
RA   AVIS J.M., ALLAIN F.H.-T., HOWE P.W.A., VARANI G., NAGAI K.,
RA   NEUHAUS D.;
RL   J. MOL. BIOL. 257:398-411(1996).
RN   [9]
RP   MUTAGENESIS, AND DETAILED STUDIES OF RNA-BINDING.
RX   MEDLINE; 92007796.
RA   JESSEN T.-H., OUBRIDGE C., TEO C.H., PRITCHARD C., NAGAI K.;
RL   EMBO J. 10:3447-3456(1991).
CC   -!- FUNCTION: BINDS STEM LOOP II OF U1 SNRNA. IT IS THE FIRST SN-RNP
CC       TO INTERACT WITH PRE-MRNA. THIS INTERACTION IS REQUIRED FOR THE
CC       SUBSEQUENT BINDING OF U2 SN-RNP AND THE U4/U6/U5 TRI-SN-RNP.
CC   -!- SUBUNIT: BELONGS TO THE SPLICEOSOME WHERE IT IS ASSOCIATED WITH
CC       SN-RNP U1.
CC   -!- SUBCELLULAR LOCATION: NUCLEAR.
CC   -!- SIMILARITY: BELONGS TO THE U1 A/B" FAMILY.
CC   -!- SIMILARITY: CONTAINS 2 RNA RECOGNITION MOTIFS (RNP).
DR   EMBL; M60784; G340052; -.
DR   EMBL; M60779; G340052; JOINED.
DR   EMBL; M60780; G340052; JOINED.
DR   EMBL; M60781; G340052; JOINED.
DR   EMBL; M60782; G340052; JOINED.
DR   EMBL; M60783; G340052; JOINED.
DR   EMBL; X06347; G37541; -.
DR   PIR; JQ1528; JQ1528.
DR   PDB; 1NRC; 31-JAN-94.
DR   MIM; 182285; -.
DR   PROSITE; PS00030; RNP_1.
KW   NUCLEAR PROTEIN; RNA-BINDING; RIBONUCLEOPROTEIN; REPEAT;
KW   SPLICEOSOME; 3D-STRUCTURE.
FT   DOMAIN       12     17       RNA-BINDING (RNP2) (BY SIMILARITY).
FT   DOMAIN       52     59       RNA-BINDING (RNP1) (BY SIMILARITY).
FT   DOMAIN      210    215       RNA-BINDING (RNP2) (BY SIMILARITY).
FT   DOMAIN      244    251       RNA-BINDING (RNP1) (BY SIMILARITY).
FT   REPEAT        1     89
FT   REPEAT      199    282
FT   MUTAGEN      11     11       T->V: ABOLISHES RNA-BINDING.
FT   MUTAGEN      13     13       Y->F: SUBSTANTIALLY REDUCES RNA-BINDING.
FT   MUTAGEN      15     15       N->V: ABOLISHES RNA-BINDING.
FT   MUTAGEN      16     16       N->V: SUBSTANTIALLY REDUCES RNA-BINDING.
FT   MUTAGEN      52     52       R->Q: ABOLISHES RNA-BINDING.
SQ   SEQUENCE   282 AA;  31279 MW;  22427816 CRC32;
     MAVPETRPNH TIYINNLNEK IKKDELKKSL YAIFSQFGQI LDILVSRSLK MRGQAFVIFK
     EVSSATNALR SMQGFPFYDK PMRIQYAKTD SDIIAKMKGT FVERDRKREK RKPKSQETPA
     TKKAVQGGGA TPVVGAVQGP VPGMPPMTQA PRIMHHMPGQ PPYMPPPGMI PPPGLAPGQI
     PPGAMPPQQL MPGQMPPAQP LSENPPNHIL FLTNLPEETN ELMLSMLFNQ FPGFKEVRLV
     PGRHDIAFVE FDNEVQAGAA RDALQGFKIT QNNAMKISFA KK
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